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在10%酪蛋白和5%明胶提供全蛋白氨基酸的基础上,用晶体氨基酸赖氨酸调节7个饲料赖氨酸水平(0.85%～2.95%),并以晶体天冬氨酸和谷氨酸(1∶1)补偿调节百分比;其它饲料氨基酸分别用晶体氨基酸调节到40%的日本沼虾肌肉蛋白中各氨基酸含量的水平,配制7种精制试验饲料,喂养初始体重为0.13 g的幼日本沼虾7周。结果表明,用不同试验饲料喂养日本沼虾7周后,虾的存活率组间无显著差异(P>0.05)。饲料赖氨酸水平对虾体增重有显著影响(P<0.05)。虾体增重随饲料赖氨酸上升到1.90%不断增大,但超过此值后不再有显著变化。摄食饲料赖氨酸水平为1.55%～2.60%的试验组的饲料效率显著高于摄食饲料赖氨酸水平为0.85%的试验组。以虾体增重为指标,经折线模型回归分析,得出日本沼虾对赖氨酸的需求量为:占干饲料的1.91%,占饲料蛋白的5.41%。并以此为基准,以肌肉必需氨基酸组成比为参考,估算了其它各必需氨基酸的需要量分别为:精氨酸1.83%、组氨酸0.52%、异亮氨酸1.13%、亮氨酸1.80%、蛋氨酸0.66%、苯丙氨酸0.99%、苏氨酸0.81%、色氨酸0.27%、缬氨酸1.11%。
Seven dietary lysine levels (0.85% -2.95%) were modulated with the crystalline amino acid lysine based on the provision of the full proteinogenic amino acids at 10% casein and 5% gelatin. Crystalline aspartic acid and glutamic acid (1: 1) to adjust the percentage of adjustment; other feed amino acids were crystal amino acids were adjusted to 40% of the Japanese marsh shrimp muscle protein levels of each other to prepare seven kinds of refined test feed, feed the initial weight of 0.13 g Young Nipponbare Shrimp for 7 weeks. The results showed that there was no significant difference in the survival rate of shrimp (P> 0.05) after seven weeks feeding with different experimental diets. Dietary lysine levels had significant effects on body weight gain (P <0.05). Shrimp body weight gain with feed lysine increased to 1.90% increasing, but beyond this value no significant changes. The feed efficiency of the experimental group fed with dietary lysine levels of 1.55% -2.60% was significantly higher than that of the experimental group fed diet with 0.85% lysine level. The body weight of shrimp as an indicator, by the polyline model regression analysis, the demand for lysine Japan Macrobrachium was: accounting for 1.91% of dry feed, 5.41% of feed protein. And based on this, the essential amino acid composition ratio of muscle as a reference, the estimated requirements of other essential amino acids were: arginine 1.83%, histidine 0.52%, isoleucine 1.13%, leucine 1.80 0.96% methionine, 0.99% phenylalanine, 0.81% threonine, 0.27% tryptophan and 1.11% valine.