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目的研究姜黄素(Cur)对人胃腺癌细胞(SGC-7901)增殖抑制和诱导凋亡作用。方法采用CCK-8检测不同浓度Cur对SGC-7901细胞的24、48、72 h的抑制作用;TUNEL检测各浓度Cur作用24h后SGC-7901细胞凋亡的发生率;Western blot法检测各浓度Cur作用SGC-7901细胞后剪切半胱氨酰天冬氨酸酶-3(cleaved-caspase-3)、剪切半胱氨酰天冬氨酸酶-9(cleaved-caspase-9)、Bax和Bcl-2蛋白量的表达。结果与对照组比较,50、100、200μmol/L Cur作用胃癌SGC-7901细胞后吸光度下降(P<0.05),同时药物剂量越高,作用时间越长,吸光度越低(P<0.01);12、24、48h IC50分别为156.51、86.88、35.32μmol/L;Cur能上调SGC-7901细胞中cleaved-caspase-3,cleaved-caspase-9和Bax蛋白的表达(P<0.05),而同时能下调Bcl-2蛋白的表达(P<0.05)。结论姜黄素具有抑制人胃癌SGC-7901细胞增殖,诱导其凋亡的作用;其抗肿瘤作用机制与激活caspase-3凋亡通路及Bcl-2蛋白家族相关。
Objective To study the effects of Cur on the proliferation and apoptosis of human gastric adenocarcinoma cell line SGC-7901. Methods CCK-8 was used to detect the inhibitory effects of different concentrations of Cur on SGC-7901 cells at 24, 48, and 72 h. TUNEL was used to detect the apoptosis rate of SGC-7901 cells treated with Cur at various concentrations for 24 h. Curcumin After cleaved-caspase-3, cleaved-caspase-9, Bax and caspase-9 in SGC-7901 cells, Expression of Bcl-2 protein. Results Compared with the control group, the absorbance of 50, 200 and 200μmol / L Cur treated gastric cancer SGC-7901 cells decreased (P <0.05), and the higher the dose of drug, the longer the action time and the lower the absorbance (P <0.01); , And the IC50 values at 24 and 48h were 156.51, 86.88 and 35.32μmol / L, respectively. Cur increased the expression of cleaved-caspase-3, cleaved-caspase-9 and Bax in SGC-7901 cells (P < Bcl-2 protein expression (P <0.05). Conclusion Curcumin can inhibit the proliferation and induce the apoptosis of human gastric cancer SGC-7901 cells. The anti-tumor mechanism is related to the activation of caspase-3 apoptosis pathway and Bcl-2 protein family.