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Second-harmonic generation(SHG)microscopy is a recently developed nonlinear optical imaging modality for imaging tissue structures with submicron resolution and is a potent tool for visualizing pathological effects of diseases.In this letter,we present our investigation on the influence of van Gieson’s(VG) alcoholic picrofuchsin staining on SHG in typeⅠcollagen(from tendon-rich C57BL/6).Multi-channel imaging and spectra analysis show that the strong SHG signal produced in fresh collagen typeⅠfiber has been greatly suppressed after VG staining,which indicates that staining may induce the structural or characteristic changes of SHG-dependent crystal formed by collagen constituents,such as glycine,proline, and hydroxyproline.
Second-harmonic generation (SHG) microscopy is a recently developed nonlinear optical imaging modality for imaging tissue structures with submicron resolution and is a potent tool for visualizing pathological effects of diseases. In this letter, we present our investigation on the influence of van Gieson’s ( VG) alcoholic picrofuchsin staining on SHG in type Icollagen (from tendon-rich C57BL / 6). Multi-channel imaging and spectra analysis show that the strong SHG signal produced in fresh collagen type I have been greatly suppressed after VG staining, which indicates that staining may induce the structural or characteristic changes of SHG-dependent crystal formed by collagen constituents, such as glycine, proline, and hydroxyproline.