目的探讨急性非淋巴细胞白血病（ＡＮＬＬ）中降钙素基因（ＣＴ基因）的甲基化状况及其意义。方法利用限制性内切酶和聚合酶链反应（ＰＣＲ）技术。结果２５例ＡＮＬＬ患者中１８例检测到ＣＴ基因高度甲基化，阳性率７２％，而且实验的敏感性至少达到１０－３水平，结论ＣＴ基因高度甲基化较普遍地存在于ＡＮＬＬ，可作为ＡＮ－ＬＬ恶性细胞的分子标志，用于ＡＮＬＬ患者治疗后微小残留病（ＭＲＤ）的检测，以利于预测复发、指导治疗和评价疗效。 Objective To investigate the methylation status of calcitonin gene (CT gene) in acute non-lymphocytic leukemia (ANLL) and its significance. Methods Using restriction endonucleases and polymerase chain reaction (PCR) techniques. Results Among the 25 patients with ANLL, CT gene hypermethylation was detected in 18 of 18 patients and the positive rate was 72%. The sensitivity of the experiment was at least 10-3. Conclusion The highly methylated CT gene is more commonly found in ANLL and can be used as The molecular markers of AN-LL malignant cells are used for the detection of minimal residual disease (MRD) after ANLL patients are treated to predict the recurrence, guide the treatment and evaluate the curative effect.