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为探究解淀粉芽孢杆菌在葡萄叶片上的定殖能力,本研究先后采用利福平和链霉素对解淀粉芽孢杆菌N24进行双抗诱导,获得了菌落特征与野生菌相同且抗药性稳定的双抗菌株(抗利福平320μg/m L、抗链霉素150μg/m L)。此双抗菌株在葡萄叶片上的定殖试验结果表明:浓度为1×104cfu/m L和1×106cfu/m L的菌液喷施叶面后,5~10 d菌量快速增加,10 d达到最高峰,此时其定殖和繁殖能力最强,尤其是起始菌液浓度为1×106cfu/m L时,叶片上的定殖量可达1.7×103cfu/g。浓度为1×108cfu/m L的菌液喷施叶面时,在喷雾后5 d菌量达到最高峰。综合来看,生防菌N24的最适喷施菌液浓度为1×106cfu/m L。添加增效因子后有利于双抗菌株在葡萄叶片表面的定殖,其中,添加壳聚糖的效果最好,其次为苏氨酸。添加磷酸氢二钾后的菌量与对照相比,没有显著差别。
In order to explore the ability of Bacillus amyloliquefaciens to colonize grape leaves, we used rifampicin and streptomycin to induce double resistance of Bacillus amyloliquefaciens N24, and obtained the same double colony with the same antibiotic-resistant colonies Antibacterial strain (anti-rifampicin 320μg / m L, anti-streptomycin 150μg / m L). The results of colonization test on grapevine leaves showed that the bacterial counts increased rapidly from 5 to 10 days after spraying at concentrations of 1 × 104 cfu / m L and 1 × 106 cfu / m L for 10 d Reached the peak, at this time its ability to colonize and reproduce the strongest, especially when the initial bacterial concentration of 1 × 106cfu / m L, the colonization on the leaf up to 1.7 × 103cfu / g. The concentration of 1 × 108cfu / m L of the bacterial spray foliar surface, 5 days after spraying reached the highest peak. Taken together, the optimum biocontrol bacterial strain N24 spray liquid concentration of 1 × 106cfu / m L. Adding the synergistic factor is beneficial to the colonization of the double-resistant strains on the surface of the grape leaves. Among them, the effect of adding chitosan is the best, followed by the threonine. After adding dipotassium hydrogen phosphate bacteria compared with the control, no significant difference.