植物名称:夹竹桃(Nerium indicum) 材料类别:幼嫩叶片培养条件:叶片用0.1％升汞消毒后剪成0.5～1.0cm大小的组织块,在MS附加1mg/1IAA,0.5mg/1 2,4-D,0.5mg/1KT固体培养基中培养。培养温度为28℃,每天光照10小时,光照度为4000lx。生长和分化情况:培养7天左右在叶片伤口处产生白色的愈伤组织,1个月左右愈伤组织长至直径1～2cm。这时可以看到两种不同类型的愈伤组织,一种是乳白色的,开始分化出根系,但一直未 Plant name: Nerium indicum Material type: Young leaves Culture conditions: The leaves were cut into 0.5 ~ 1.0cm tissue blocks after sterilizing with 0.1% mercuric chloride, and MS was supplemented with 1mg / 1 IAA, 0.5mg / 1 2 , 4-D, 0.5mg / 1KT solid medium. Incubation temperature is 28 ℃, light per day 10 hours, illumination 4000lx. Growth and differentiation: about 7 days in the leaf wound at the white callus, callus around 1 month up to a diameter of 1 ~ 2cm. Two different types of calluses can be seen at this time, one of which is milky white and begins to differentiate into roots, but has not been