【摘 要】
:
SARS coronavirus is an RNA virus whose replication is error-prone, which provides possibility for escape of host defenses, and even leads to evolution of new vi
【机 构】
:
State Key Laboratory for Agrobiotechnology,Sino Biotech Co.
论文部分内容阅读
SARS coronavirus is an RNA virus whose replication is error-prone, which provides possibility for escape of host defenses, and even leads to evolution of new viral strains during the passage or the transmission. Lots of variations have been detected among different SARS-CoV strains. And a study on these variations is helpful for development of efficient vaccine. Moreover, the test of nucleic acid characterization and genetic stability of SARS-CoV is important in the research of inactivated vaccine. The whole genome sequences of two SARS coronavirus strains after passage in Vero cell culture were determined and were compared with those of early passages, respectively. Results showed that both SARS coronavirus strains have high genetic stability, although nearly 10 generations were passed. Four nucleotide variations were observed between the second passage and the 11th passage of Sino1 strain for identification of SARS inactivated vaccine. Moreover, only one nucleotide was different between the third passage and the 10th passage of Sino3 strain for SARS inactivated vaccine. Therefore, this study suggested it was possible to develop inactivated vaccine against SARS-CoV in the future.
其他文献
目的:比较远端胃切除术后毕Ⅰ式与Roux-en-Y消化道重建的远期疗效。方法回顾性分析2000年6月至2010年6月间在广州医科大学附属肿瘤医院接受根治性远端胃大部分切除151例胃癌患
Bacteriophages infected different serotypes of Klebsiella were isolated from sewage. Among them, a heat-stable polysaccharide depolymerase enzyme which could de
To observe the effects of basic fibroblast growth factor (bFGF) on human adenoid cystic carcinoma ACC-2 cell line proliferation and ERK, cyclin D1/p21waf/ciplsi
利用有限元方法建立了符合静压桩实际的有限元模型.该模型采用有限变形理论和自适应网格技术,并通过桩土界面的接触和施加位移荷载来实现压桩过程.运用计算得到的静压桩残余
采用实验室规模喷淋脱硝装置对ClO2溶液去除NO的效果及影响因素进行探讨,通过脱硝产物的测定对ClO2溶液去除NO的能力及机理进行分析;在此基础上考察ClO2溶液对供热厂燃煤锅炉烟
简述四川"5·12"地震给当地植被和生态造成的严重危害,指出了地震后植被恢复和生态重建要遵循植被的生态完整性、植被的异质性、物种间的生态交互性和生态系统的排外性的原则
An in vivo expression technology (IVET) was applied to screen S. flexneri2a genes induced after invasion of epithelial cells, and virulence-related genes were f
2008年5月12日,四川省阿坝州汶川县发生里氏8.0级强烈地震.地震导致龙门山断裂带上约10万平方公里区域内的大量人员伤亡.伤者以肢体损伤为主,多数还合并有其他组织器官的损伤
Spermatogonial stem cell (SSC) transplantation is a novel technique by which testicular cells from normal, transgenic or mutant donor are introduced into the se
针对丹东市采暖期SO_2污染的实际情况及气象因子的关系,建立了逐步回归、偏最小二乘回归,主成分回归和BP神经网络等4种常用的大气污染预报模式,并在实际预报中进行了模拟、试