论文部分内容阅读
给妊娠金黄地鼠灌服过量维生素Ａ酸后，在不同时间剖腹取胎，石蜡包埋、连续切片。用免疫组织化学方法，对神经上皮及其基膜、脊索和神经管周围间充质中的基质粘着分子——纤维粘连蛋白和层粘连蛋白进行定性、定位和半定量观察。结果显示，实验组各组胚胎的纤维粘连蛋白和层粘连蛋白的免疫组织化学染色均有不同程度的减弱。说明过量维生素Ａ酸可使神经上皮及其基膜和周围间充质中的纤维粘连蛋白和层粘连蛋白降低，从而干扰了神经上皮细胞的正常增殖、迁移、生理性死亡和细胞粘着过程。这是维生素Ａ引起神经管畸形的一个重要途径。
Giving pregnant golden hamster with excess vitamin A acid, cesarean section taken at different times, embedded in paraffin, serial sections. Immunohistochemistry was used to characterize, locate and semi-quantitatively observe the matrix adhesion molecules-fibronectin and laminin in the mesenchyme of the neural epithelium and its basement membrane, the chordae and the neural tube. The results showed that immunohistochemical staining of fibronectin and laminin in different groups of embryos in different groups decreased to some extent. The results showed that excess retinoic acid reduced the expression of fibronectin and laminin in the neuroepithelial and its basement membrane and surrounding mesenchyme, which interferes with the normal proliferation, migration, physiological death and cell adhesion of neuroepithelial cells. This is an important way of vitamin A induced neural tube defects.