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以逐代人工耐盐性筛选的栽培和野生大豆杂交组合(Lee68×N23227)F5代株系3060为父本,以耐盐栽培大豆Lee68品种为母本或轮回亲本,配置回交组合(Lee68×3060,编号C)得到的不同单株后代株系(BC1F2)为试验材料,采用苗期耐盐系数、相对生长速率、干物质积累量等指标,对它们的耐盐性进行了分析和评价,并采用相对电解质渗漏率、硫代巴比妥酸反应物(TBARS)含量、叶片净光合速率(Pn)和PSⅡ最大光化学效率(Fv/Fm)对其中优良株系耐盐的生理机制进行了分析和探讨。结果表明:在盐胁迫下,该组合回交后代中C3、C10等株系的苗期耐盐性系数、相对生长速率和干物质积累量等指标均明显高于母本Lee68,接近或超过父本3060,其中C10株系尤为突出。与双亲相比,盐胁迫使C10株系幼苗根和叶中相对电解质渗漏率和TBARS含量的上升幅度,及叶片Pn和Fv/Fm值的降低幅度明显较低,且盐胁迫下的相对电解质渗漏率和TBARS含量与其对照相比,差异均未达显著水平。可认为C10株系是一种新的优良耐盐大豆种质材料。
The generation of backcrossing combinations (Lee68 × N23227) F6030 was used as the male parent and the salt-tolerant soybean cultivar Lee68 was used as female parent or reincarnated parent (Lee68 × N23227) 3060, No. C) were used as test materials to analyze the salt tolerance of BC1F2. The salt tolerance index, relative growth rate and dry matter accumulation were used to analyze their salt tolerance. The physiological mechanism of salt tolerance in the elite lines was studied using relative electrolyte leakage rate, TBARS content, leaf Pn and Fv / Fm Analysis and discussion. The results showed that under salinity stress, the index of salt tolerance, relative growth rate and dry matter accumulation of C3, C10 and other lines were significantly higher than that of female parent Lee68 in the backcross generations of this combination, close to or beyond the parent The 3060, which C10 strains are particularly prominent. Compared with the parents, the relative electrolyte leakage rate and the content of TBARS in roots and leaves of C10 plants were significantly decreased and the Pn and Fv / Fm values of leaves were significantly lower than those of the parents. The relative electrolyte under salt stress Leakage rate and TBARS content compared with the control, the difference was not reached a significant level. C10 strain can be considered as a new excellent salt tolerant soybean germplasm material.