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目的了解大连市伤寒沙门菌耐药性及分子分型特点,建立沙门菌分子特征本底信息,为今后防治工作提供科学依据。方法采用微量肉汤稀释法对46株伤寒沙门菌进行8种抗生素敏感试验;运用脉冲场凝胶电泳(PFGE)方法对46株伤寒沙门菌进行分子分型及聚类分析。结果 46株伤寒沙门菌对萘啶酸(NAL)100.00%敏感,对氯霉素(CHL)和甲氧苄啶/磺胺甲噁唑(TMP/SMZ)耐药率为4.35%,对庆大霉素(GEN)耐药率为47.83%,发现多重耐药株1株;BioNumerics分析结果显示,46株伤寒沙门菌共产生30种PFGE带型,有7株表现为同一PFGE型别。结论大连地区存在耐庆大霉素的伤寒沙门菌;PFGE结果表明这些菌株存在遗传多态性,并有优势菌株的存在。
Objective To understand the characteristics of Salmonella typhi resistance and molecular typing in Dalian and to establish the background information of molecular characteristics of Salmonella to provide a scientific basis for future prevention and treatment work. Methods 46 strains of Salmonella typhi were subjected to antibiotic susceptibility test by micro broth dilution method. The molecular typing and cluster analysis of 46 strains of Salmonella typhi were carried out by pulse field gel electrophoresis (PFGE). Results 46 strains of Salmonella typhi were sensitive to 100% Nalidixic acid (NAL), 4.35% resistant to chloramphenicol (CHL) and trimethoprim / sulfamethoxazole (TMP / SMZ) The gene resistance rate of GEN was 47.83%, and one multi-drug resistant strain was found. BioNumerics analysis showed that 46 Salmonella typhi strains produced 30 PFGE bands and 7 strains showed the same PFGE type. Conclusion There is gentamicin-resistant Salmonella typhi in Dalian. PFGE results show that these strains have genetic polymorphism and predominant strains exist.