The EBD-SIM (evidence-based design, simulation) framework is a conceptual framework developed to integrate the use of lighting simulation in the EBD process to provide a holistic performance evaluation method. A real-time case study, executed in a fully operational office building, is used to demonstrate the framework’s performance. The case study focused on visual comfort analysis. The objective is to demonstrate the applicability of the developed EBD-SIM framework using correlations between current visual comfort metrics and actual human perception as evaluation criteria. The data were collected via simulation for visual comfort analysis and via questionnaires for instantaneous and annual visual comfort perception. The study showed that for user perception, the most crucial factor for visual comfort is the amount of light on a task area, and simple metrics such as Eh-room and Eh-task had a higher correlation with perceived visual comfort than complex performance metrics such as Daylight Autonomy (DA). To improve the design process, the study suggests that, among other things, post-occupancy evaluations (POEs) should be conducted more frequently to obtain better insight into user perception of daylight and subsequently use new evidence to further improve the design of the EBD-SIM model.