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本文采用水平淀粉凝胶电泳技术对分布于北京、山东和大连4个野生大豆天然居群共计120个体进行了等位酶水平遗传多样性分析.7个酶系统13个等位酶位点的检测表明,该地区野生大豆天然居群遗传变异水平较高,多态位点比率P=69.20,等位基因平均数A=1.77,平均期望杂合度He=0.133,居群间有较明显的遗传分化,基因分化系数G_(st)=0.391,即有39.1%的遗传变异存在于居群间.本文结果表明该地区遗传固定指数F偏小,居群异交率较高.4个居群遗传多样性明显高于日本野生大豆研究结果,而与韩国野生大豆遗传变异水平相近,进一步证明我国为野生大豆遗传变异中心.
In this study, 120 isolates of four wild soybean populations distributed in Beijing, Shandong and Dalian were analyzed for their genetic diversity by horizontal starch gel electrophoresis (PAGE) .13 alleles The results showed that the natural populations of wild soybean in this area had higher genetic variation, the ratio of polymorphic loci P = 69.20, the average number of alleles A = 1.77, the average expected heterozygosity He = 0.133, and more obvious genetic differentiation among populations , The genetic differentiation coefficient G st (st) = 0.391, that is 39.1% of the genetic variation exists among the populations.The results show that the genetic fixed index F is small and the population outcrossing rate is high.The genetic diversity of four populations The results were significantly higher than those of Japanese wild soybean and similar to the genetic variation of wild soybean in South Korea, further demonstrating that our country is the center of genetic variation of wild soybean.