目的:建立高效液相色谱法测定宜宾地区蒲公英中木樨草素的含量。方法:采用Kromasil C_(18)柱(200 mm×4.6 mm,5μm),流动相为乙腈-0.1%磷酸溶液(28:72),检测波长为349 nm,流速为1.0 ml·min~(-1)。结果:木樨草素的线性范围为0.515～6.180μg·ml~(-1)(r=0.999 8),平均回收率为102.10%(RSD=1.25%)。结论:该方法准确、可靠,可用于宜宾地区蒲公英的质量控制。 Objective: To establish a HPLC method for the determination of tardinin in dandelion in Yibin area. Methods: Kromasil C 18 column (200 mm × 4.6 mm, 5 μm) was used. The mobile phase was acetonitrile - 0.1% phosphoric acid solution (28:72). The detection wavelength was 349 nm and the flow rate was 1.0 ml · min -1 ). Results: The linear range was 0.515 ~ 6.180μg · ml -1 (r = 0.999 8). The average recovery was 102.10% (RSD = 1.25%). Conclusion: The method is accurate and reliable and can be used for quality control of dandelion in Yibin area.