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为探讨外源一氧化氮(NO)供体硝普钠(SNP)对铜(Cu2+)胁迫的缓解效应,该试验以小桐子幼苗为材料,先通过小桐子幼苗根茎生长指标的变化筛选出后续实验适宜的SNP(0.2mmol/L)和Cu2+浓度(90mmol/L),再进一步考察SNP预处理对Cu2+胁迫下幼苗脯氨酸(Pro)和丙二醛(MDA)的含量及抗氧化酶活性的影响。结果显示:(1)Cu2+处理能诱导小桐子幼苗叶片中Pro和MDA含量显著升高,且过氧化氢酶(CAT)、超氧物歧化酶(SOD)、愈创木酚过氧化物酶(POD)及抗坏血酸专一性过氧化物酶(APX)的活性增强。(2)用SNP预处理能显著提高Cu2+胁迫下幼苗叶片Pro的含量,进一步增强叶片中CAT、SOD、POD和APX活性,降低MDA的含量。研究表明,0.2mmol/L的SNP能够通过提高小桐子幼苗的抗氧化酶活性和游离脯氨酸含量来增强其抗氧化胁迫能力,从而显著缓解Cu2+胁迫对幼苗造成的氧化伤害,维持其正常生长发育。
In order to explore the alleviating effect of SNP on copper (Cu2 +) stress, this experiment took Jatropha curcas seedlings as material, screened out the changes of rhizome growth index of Jatropha curcas seedlings The optimum SNP (0.2 mmol / L) and Cu2 + concentration (90 mmol / L) were used to investigate the effect of SNP pretreatment on the content of Proline and malondialdehyde (MDA) and the activity of antioxidant enzymes in seedlings under Cu2 + stress Impact. The results showed that: (1) Cu2 + treatment could induce the increase of Pro and MDA in the leaves of Jatropha curcas seedlings, and the contents of CAT, superoxide dismutase (SOD), guaiacolperoxidase POD) and ascorbate-specific peroxidase (APX) activity increased. (2) Pretreatment with SNP could significantly increase the content of Pro in seedling leaves under Cu2 + stress, and further increase the activity of CAT, SOD, POD and APX in leaves and decrease the content of MDA. The results showed that 0.2 mmol / L SNP can enhance the antioxidant ability of Jatropha curcas seedlings by increasing its antioxidant enzyme activity and free proline content, thereby significantly reducing the oxidative damage caused by Cu2 + stress to seedlings and maintaining their normal growth development.