ELISA是目前常用的血清学诊断方法之一,但多采用可溶性抗原。本文报病了以培养的利什曼完整前鞭毛期原虫经福尔马林处理后,根据需要的原虫数量,直接放在聚苯乙烯板上吸附,然后按ELISA常规操作方法进行抗体检测,发现完整前鞭毛期原虫抗原对黑热病有和可溶性抗原相同的诊断效果。 ELISA is one of the commonly used serological diagnostic methods, but the use of soluble antigen. In this paper, the report of the Leishman training complete pre-flagella protracted by formalin treatment, according to the number of protozoa required, directly on the polystyrene board adsorption, and then by ELISA conventional method of antibody detection and found that The complete pre-flagella protozoal antigen has the same diagnostic efficacy as kala-azar for soluble antigens.