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目的探讨载脂蛋白E(ApoE)基因多态性与糖尿病肾病(DN)的关系。方法应用聚合酶链反应-限制性片断长度多态性技术检测218例2型糖尿病患者[正常白蛋白尿(DNO)组41例,微量白蛋白尿(DN1)组129例,临床白蛋白尿(DN2)组48例]和102名健康对照者(NC)的ApoE基因多态性。结果 (1)DN1组、DN2组及DN组(DN1+DN2组)ApoE 2/3基因型和ε2等位基因频率均高于DN0组(P均<0.05)。(2)ApoE 2/3基因型为DN发生的危险因素(P<0.05)。(3)ε4等位基因频率与TC负相关(P<0.05)。结论 2型糖尿病患者中,ApoE 2/3基因型可能是DN发生的危险因素,ε4等位基因可能是高TC血症的保护因素。
Objective To investigate the relationship between apolipoprotein E (ApoE) gene polymorphism and diabetic nephropathy (DN). Methods The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the expression of interleukin-2 in 218 patients with type 2 diabetes mellitus (normal albuminuria group, 41 patients), microalbuminuria group DN2 group, 48 cases] and 102 healthy controls (NC). Results (1) The frequencies of ApoE 2/3 genotypes and ε2 alleles in DN1, DN2 and DN groups (DN1 + DN2) were higher than those in DN0 group (all P <0.05). (2) ApoE 2/3 genotype is a risk factor for DN (P <0.05). (3) The frequency of ε4 allele was negatively correlated with TC (P <0.05). Conclusion ApoE 2/3 genotype may be a risk factor for DN in patients with type 2 diabetes. The ε4 allele may be a protective factor in hypercholesterolemia.