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1实验材料1.1实验动物及细胞C57BL6J小鼠,雄性,体重16～18g,购自中国医学科学院实验动物中心。L929细胞,由中国医学科学院生物技术研究所甄永苏教授惠赠。1.2药品与试剂培养雪莲总黄酮(水母雪莲红色系固体培养细胞)的碱洗部分,中科院植物所提供;巯基乙醇酸钠(Difoco);胰酶(Sigm
1 Experimental Materials 1.1 Experimental Animals and Cells C57BL6J mice, male, weighing 16-18 g, were purchased from the Experimental Animal Center of the Chinese Academy of Medical Sciences. L929 cells were gifted by Prof. Yongsheng Yong from Institute of Biotechnology, Chinese Academy of Medical Sciences. 1.2 Drugs and reagents Alkaline washes of total flavonoids of Saussurea (Saussurea involucrata) were provided by the plant of the Chinese Academy of Sciences; Difoco sodium (Difoco); Pancreatin (Sigm