目的了解基因芯片检测人乳头状瘤病毒(HPV)21种亚型感染的临床应用价值。评价导流杂交基因芯片技术(凯普导流杂交)HPV DNA检测法(HybriMax)在女性生殖道HPV感染检测中的临床效用,初步探讨最常见的HPV基因型。方法利用标记有生物素的HPV通用引物进行PCR扩增,扩增产物变性后与固定在尼龙膜上21种HPV特异性分型探针进行反向斑点杂交检测HPV亚型。结果 591例患者标本中,基因芯片阳性率69.2%,其中单一亚型感染266例,重叠感染143例。共检出亚型20种。结论基因芯片一次试验可联合检测多种HPV亚型感染并分型,可研究HPV感染型别的分布,提高由HPV引起的肿瘤防治水平。 Objective To understand the clinical value of microarray in detecting 21 subtypes of human papillomavirus (HPV) infection. To evaluate the clinical utility of HybriMax in detection of HPV infection in female genital tract by flow-through hybridization microarray (CAP), and to explore the most common HPV genotype. Methods HPV universal primers labeled with biotin were used for PCR amplification. The HPV subtypes were detected by reverse dot blot hybridization with 21 HPV-specific typing probes immobilized on nylon membrane after denatured. Results Among the 591 patients, the positive rate of gene chip was 69.2%, of which 266 were single subtype infection and 143 were overlap infection. A total of 20 subtypes were detected. Conclusion Gene chips can be used to detect and classify multiple HPV subtypes in one test. The distribution of HPV infection types can be studied and the level of tumor prevention and control caused by HPV can be increased.