论文部分内容阅读
目的:研究冠心丹参处方中,分别除去降香总萜和降香总黄酮,或是加入丹参酮,研究其对抗心肌细胞缺氧/复氧(A/R)损伤的保护作用,探讨各组分对药效的贡献。方法:取培养4 d的大鼠乳鼠心肌细胞,随机分为:对照组(Cont组)、(1)A/R组、(2)1%的DSMO对照组、(3)冠心丹参组、(4)～(6)降香总萜与/和降香总黄酮阴性组、或是冠心丹参处方中分别加入终浓度为1,2,4 mg的丹参酮;检测细胞存活率、超氧化物歧化酶(SOD)、过氧化氢酶(catalase)、谷胱甘肽过氧化物酶(GSH-Px)活性、丙二醛(MDA)含量及孵育液乳酸脱氢酶(LDH)活性。结果:在冠心丹参处方中分别除去降香总萜与/和降香总黄酮后,样品对抗心肌细胞的A/R的作用显著减弱,尤其以反映细胞抗脂质氧化状态指标SOD,catalase,GPx活性以及MDA含量更为明显;而加入丹参酮后,其对抗心肌细胞A/R的作用有不同程度的增强,尤其以反映细胞存活状态指标细胞存活率以及LDH更为明显,以2 mg丹参酮最为理想。结论:冠心丹参处方中在对抗心肌细胞A/R损伤的作用上,降香总萜和降香总黄酮不可缺如;加入2 mg丹参酮可增强其心肌保护作用。
OBJECTIVE: To study the protective effects of Danshen Danshen (Radix Angelicae Sinensis) and Rhizoma Ligustici Flavonoids (Rhizoma Anemarrhena astigmatitus), or Tanshinone (Rhodanin) on myocardial cell hypoxia / reoxygenation injury (A / R) Contribution to efficacy. Methods: The neonatal rat cardiomyocytes cultured for 4 days were randomly divided into control group (Cont group), (1) A / R group, (2) 1% DSMO control group, (3) , (4) ~ (6), total flavonoids and / or flavonoids, or Guanxin Danshen were added to the final concentration of 1, 2, 4 mg of tanshinone; cell viability was measured, SOD, catalase, GSH-Px and malondialdehyde (MDA) contents and lactate dehydrogenase (LDH) activity in the incubation solution were measured. Results: The effect of A / R on cardiomyocytes was significantly weakened after removing the total alkaloids and / or the total flavonoids in Danshen Danshen decoction. In particular, the effect of A / R on the anti-lipoxygenase activity index (SOD, catalase, GPx activity and MDA content were more obvious. When added tanshinone, the effect of ATR on cardiomyocyte A / R was enhanced to some extent, especially the cell viability and LDH, which reflect the index of cell survival, ideal. CONCLUSION: In the treatment of A / R injury of cardiomyocytes in Danshen Danshen decoction, the total flavonoids of Atractylodes macrocephala and Rhodiola flavonoids are indispensable. The addition of 2 mg tanshinone can enhance the myocardial protective effect.