论文部分内容阅读
研究了一种新型超分子纳米药物载体的制备方法及其药物释放性能.将α-环糊精(α-CD)穿入肉桂酸改性的PEG分子链形成包含复合物(inclusion complex,IC),通过超分子自组装成为纳米粒子.将抗肿瘤药物阿霉素负载到纳米粒子中,研究药物释放行为及其对肿瘤细胞的抑制效果.以核磁共振(1HNMR)、X射线衍射(XRD)、紫外吸收光谱(UV)、动态光散射(DLS)、扫描电镜(SEM)、透射电镜(TEM)和原子力显微镜(AFM)表征了纳米粒子的结构和形貌,用激光共聚焦显微镜(Confocal)研究了载药纳米粒子在细胞内的分布及其对肿瘤细胞的抑制效果.结果显示超分子纳米粒子具有很好的生物相容性和药物缓释作用,载药纳米粒子对肿瘤细胞具有很好的杀伤效果.
A new type of supramolecular nanophase drug carrier was prepared and its drug release properties were studied.It was found that α-cyclodextrin (α-CD) penetrated cinnamic acid-modified PEG molecular chain to form an inclusion complex (IC) , And assembled into nanoparticles by supramolecular self-assembly.The doxorubicin loaded into the nanoparticle was used to study drug release behavior and its inhibitory effect on tumor cells.H NMR, X-ray diffraction (XRD) The structure and morphology of the nanoparticles were characterized by UV, DLS, SEM, TEM and AFM. The morphology of the nanoparticles was characterized by confocal microscopy The intracellular distribution of drug-loaded nanoparticles and their inhibitory effect on tumor cells were investigated. The results showed that the supramolecular nanoparticles had good biocompatibility and sustained drug release. The drug-loaded nanoparticles had good cytotoxicity against tumor cells Killing effect.