论文部分内容阅读
目的建立反相高效液相色谱法同时测定五味子中6种木脂素含量的方法,并应用此方法检测南、北五味子及3种市售成药:参芪五味子片、安神补心片、安神补心胶囊中此6种木脂素的含量。方法采用SHIMADZU VP-ODS(4.6 mm×150 mm i.d,5μm)色谱柱,以乙腈-水为流动相,进行梯度洗脱,流速0.5mL/min,检测波长220 nm,柱温30℃。结果五味子醇甲、五味子醇乙、五味子酯甲、五味子甲素、五味子乙素和五味子丙素分离度良好,分别在0.243~2.430μg、0.087~0.870μg、0.060~0.600μg、0.093~0.930μg、0.246~2.460μg、0.063~0.630μg的进样量范围内有良好的线性关系,平均加样回收率分别为100.93%、100.59%、99.78%、100.43%、96.72%、102.33%,RSD值分别为2.14%、2.95%、2.26%、2.81%、1.88%、0.985%。结论本研究所建立的反相高效液相色谱法方法准确可靠、简单可行,可用于五味子中木脂素类成分的定量分析,为五味子药材及以五味子为组方药味的中成药的质量评价奠定基础。
Objective To establish a method for the simultaneous determination of six lignans in Fructus Schisandrae by reversed-phase high performance liquid chromatography (RP-HPLC) and to determine the content of six kinds of lignans in Schisandra chinensis by three methods: Shenqi Schisandra, Anshen Buxin Tablet, Heart capsule in the six kinds of lignans content. Methods The gradient elution was performed on a SHIMADZU VP-ODS column (4.6 mm × 150 mm i.d, 5 μm) using acetonitrile-water as mobile phase at a flow rate of 0.5 mL / min. The detection wavelength was set at 220 nm and the column temperature was set at 30 ℃. Results Fructus schisandrae, schisandrin B, Schisandra ester A, Schisandra chinensis, Schisandrin B and Schisandra chinensis were well separated, with good linearities of 0.243-2.430μg, 0.087-0.870μg, 0.060-0.600μg, 0.093-0.930μg, The average recoveries were 100.93%, 100.59%, 99.78%, 100.43%, 96.72% and 102.33%, respectively, with RSD values of 0.246 ~ 2.460μg and 0.063 ~ 2.14%, 2.95%, 2.26%, 2.81%, 1.88%, 0.985%. Conclusion The RP-HPLC method established in this study is accurate and reliable, simple and feasible, and can be used for the quantitative analysis of lignans in Schisandra chinensis, laying a foundation for the quality evaluation of Schisandra chinensis basis.