[Objective] To establish a quantitative method for simultaneous determination of ursolic acid and oleanolic acid in the roots of Agastache rugosa by RP-HPLC, so as to provide basis for the development of Agastache rugosa. [Method] C18 chromatographic column (4.6 mm×250 mm, 5 μm), mobile phase of acetonitrile-methanol-0.3% H3PO4 (V ∶V∶V=60∶30∶10), flow rate of 0.8 ml/min, detective wavelength of 210 nm, room temperature. [Result]The good linear relationship was obtained under the above conditions. The recovery rates of the ursolic acid and oleanolic acid were 99.6% and 98.9% with RSD of 3.12% and 1.99% respectively. The average contents of ursolic acid and oleanolic acid in the roots of Agastache rugosa were 0.138 mg/g and 0.147 mg/g respectively. [Conclusion]The method is rapid, precise and reliable, which can be used to determine the contents of ursolic acid and oleanolic acid in the roots of Agastache rugosa. This case would offer theoretical evidence for developing medicinal value of Agastache rugosa. [Objective] To establish a quantitative method for simultaneous determination of ursolic acid and oleanolic acid in the roots of Agastache rugosa by RP-HPLC, so as to provide basis for the development of Agastache rugosa. [Method] C18 chromatographic column (4.6 mm × 250 mm, 5 μm), mobile phase of acetonitrile-methanol-0.3% H3PO4 (V: V: V = 60:30:10), flow rate of 0.8 ml / min, detective wavelength of 210 nm, room temperature. The recovery of the ursolic acid and oleanolic acid were 99.6% and 98.9% with RSD of 3.12% and 1.99% respectively. The average contents of ursolic acid and oleanolic acid in the roots of Agastache rugosa were 0.138 mg / g and 0.147 mg / g respectively. [Conclusion] The method is rapid, precise and reliable, which can be used to determine the contents of ursolic acid and oleanolic acid in the roots of Agastache rugosa. would offer theoretical evidence for developing medicin al value of Agastache rugosa.