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目的研究人源与猪源猪链球菌2型gdh基因序列同参考序列的差异,为猪链球菌2型的诊断方法研究提供基础依据。方法选取猪链球菌2型患者分离株和病猪分离株各1株,根据文献和基因库中的gdh基因序列设计PCR引物,进行PCR扩增;将扩增产物纯化回收,克隆至pMD 19-T载体后进行测序,获得gdh基因序列。结果2株猪链球菌2型菌株的PCR扩增产物经电泳后,均得到1 300 bp左右的目的条带,大小与预测一致;序列分析结果显示,人源猪链球菌2型gdh基因序列GC含量为45.73%,猪源猪链球菌2型gdh基因序列GC含量为45.88%,猪链球菌2型gdh参考序列GC含量为45.81%;人源与猪源猪链球菌2型gdh序列同参考序列相比,一致性均为99.9%;人源与猪源猪链球菌2型gdh序列仅相差2个碱基。结论人源与猪源的猪链球菌2型gdh基因序列高度保守。
Objective To study the difference of gdh gene sequence between human and swine Streptococcus suis type 2 with reference sequence, and to provide a basis for the diagnosis of Streptococcus suis type 2. Methods One strain of Streptococcus suis type 2 patient and one isolate of diseased pig were selected. PCR primers were designed according to the literature and gdh gene sequences in the gene bank for PCR amplification. The amplified product was purified and cloned into pMD19- T vector after sequencing to obtain gdh gene sequence. Results The PCR products of 2 strains of Streptococcus suis serotype 2 were electrophoresed to get a band of about 1 300 bp, which was consistent with the prediction of the size. The sequence analysis showed that the genotypes of human gondii genotype 2 Content was 45.73%, the content of gdh gene of swine Streptococcus suis type 2 was 45.88%, the content of gdh was 45.81%. The gdh sequence of human and swine Streptococcus suis type 2 was the same as the reference sequence Compared to 99.9%, and the difference between human and swine Streptococcus suis type 2 gdh sequences was only 2 bases. Conclusion The genotypes of S. suis type 2 gdh gene from human and swine origin are highly conserved.