作者对肝片吸虫成虫和幼虫能量代谢的差异进行了研究。按Tielens等(1981,1984)的方法分别在体外使肝片吸虫囊蚴脱囊获得幼虫,分离12,25,52天龄的肝片吸虫。成虫从屠杀的牛、羊收集,保存在缓冲液中过夜,以清除肠内容物。将成虫和幼虫置匀浆器内,于0℃、在含 The authors studied the differences in energy metabolism between adult and larvae of Fasciola hepatica. According to the method of Tielens et al. (1981, 1984), larvae were obtained by in vitro extirpation of Hepatic actin metacercariae, and isolation of Fasciola hepatica at 12, 25 and 52 days. Adults were collected from slaughtered cattle and sheep and stored in buffer overnight to remove intestinal contents. Adult and larvae were placed homogenizer, at 0 ℃, containing