[Objective]To determine the plumbagin contents in the stems,leaves and roots of HERBA PLUMBAGINIS ZEYLANICAE under different grown conditions,and to provide test data for its domestication. [Methods]The plumbagin contents in the stems,leaves and roots of HERBA PLUMBAGINIS ZEYLANICAE were detected by RP-HPLC. [Results] Plumbagin contents in HERBA PLUMBAGINIS ZEYLANICAE grown in mellow soil and clay were higher than those grown in sandy and raw soils. Plumbagin contents in HERBA PLUMBAGINIS ZEYLANICAE applying K and N fertilizers were higher than that of P fertilizer. Plumbagin content was the maximum in HERBA PLUMBAGINIS ZEYLANICAE grown under full illumination. Plumbago contents in the roots,stems,and leaves grown under different reproduction modes showed only slight changes. [Conclusions]Considering from the angle of plumbagin content,the optimal cultivation condition for HERBA PLUMBAGINIS ZEYLANICAE was as follows: a given amount of N and K fertilizers should be applied during the growth of cutting seedlings of HERBA PLUMBAGINIS ZEYLANICAE on clay; and full illumination should be provided. Under this condition,the high-yield and highquality HERBA PLUMBAGINIS ZEYLANICAE could be obtained. [Objective] To determine the plumbagin contents in the stems, leaves and roots of HERBA PLUMBAGINIS ZEYLANICAE under different grown conditions, and to provide test data for its domestication. [Methods] The plumbagin contents in the stems, leaves and roots of HERBA PLUMBAGINIS ZEYLANICAE were detected by RP-HPLC. [Results] Plumbagin contents in HERBA PLUMBAGINIS ZEYLANICAE grown in mellow soil and clay were higher than those grown in sandy and raw soils. Plumbagin contents in HERBA PLUMBAGINIS ZEYLANICAE applying K and N fertilizers were higher than that of P Plumbago content was the maximum in HERBA PLUMBAGINIS ZEYLANICAE grown under full illumination. Plumbago contents in the roots, stems, and leaves grown under different reproduction modes showed only slight changes. [Conclusions] Considering from the angle of plumbagin content, the optimal cultivation condition for HERBA PLUMBAGINIS ZEYLANICAE was as follows: a given amount of N and K fertilizers should be applied during the gr owth of cutting seedlings of HERBA PLUMBAGINIS ZEYLANICAE on clay; and full illumination should be provided. Under this condition, the high-yield and highquality HERBA PLUMBAGINIS ZEYLANICAE could be be obtained.