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目的探讨丙型肝炎患者保护性免疫缺陷的原因。方法根据HCVC区和NS4区基因序列设计并人工合成3条合成肽,采用抗原捕捉酶联免疫吸附试验检测HCV感染者血清中抗-HCV IgG抗体轻链κ/λ比值,同时以正常人血清做对照。结果抗-HCV SP42,CP10和CP9抗体轻链的表达呈明显的偏斜;116例抗-HCV阳性者中113例(97.41％),抗-HCV IgG抗体轻链κ/λ比值偏高,所有病例追踪观察1a(其中11例抗-HCV阳性者随访2a);30例患者接受α-干扰素治疗,发现抗-HCV抗体κ/λ比值恒定不变。结论 HCV感染者抗-HCV抗体的产生不均匀性并呈稳定的克隆性。B细胞克隆优势化可能是HCV感染后机体保护性免疫缺陷的原因之一。
Objective To investigate the causes of protective immunity in patients with hepatitis C Methods Three synthetic peptides were designed and synthesized based on the sequence of HCVC and NS4. The antigen chain-capture enzyme-linked immunosorbent assay (ELISA) was used to detect the ratio of light chain κ / λ of anti-HCV IgG antibody in serum of HCV-infected patients. Control. Results The expression of anti-HCV SP42, CP10 and CP9 light chains was significantly skewed. Among 116 anti-HCV positives, 113 (97.41%) had mild light chain κ / λ ratio and all Cases were followed for 1a (11 patients were positive for anti-HCV in follow-up 2a). Thirty patients received α-interferon and found that the ratio of κ / λ of anti-HCV antibody was constant. Conclusions The HCV anti-HCV antibodies in HCV-infected patients are heterogeneously and stably cloned. The superiority of B cell clones may be one of the reasons for the protective immune deficiency in the body after HCV infection.