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目的:建立一种实时直接分析质谱法快速筛查葡萄酒中安赛蜜、阿斯巴甜、纽甜、甘草次酸、麦芽糖醇、异麦芽酮糖、木糖醇、D-山梨醇、D-甘露醇共9种甜味剂。方法:实时直接分析离子源串联三重四极杆质谱,源电压为350 V,载气为氦气,源温度350℃,样品传输速度为2 mm·s-1,采用子离子扫描模式直接分析葡萄酒样品,通过对照品与样品的二级质谱图对比进行非法添加成分的定性鉴别。结果:9种甜味剂检出限为2.5~20μg·mL-1,5批样品中检出麦芽糖醇、异麦芽酮糖、D-山梨醇及D-甘露醇,同时阐释了9种甜味剂的二级质谱裂解途径。结论:方法简单快速,每次检测时间仅需几秒,检测效率比液质联用法提高近25倍,适用于大批量葡萄酒中甜味剂类成分的快速筛查。
OBJECTIVE: To establish a real-time direct analytical mass spectrometry method for rapid screening of acesulfame potassium, aspartame, neotame, glycyrrhetinic acid, maltitol, isomaltulose, xylitol, D-sorbitol, D- Alcohol a total of 9 kinds of sweeteners. Methods: The real-time direct analysis ion mass tandem triple-quadrupole mass spectrometry with a source voltage of 350 V, a carrier gas of helium, a source temperature of 350 ° C. and a sample transmission rate of 2 mm · s-1, Samples, through the reference substance and sample secondary mass spectrometry comparison qualitative identification of illegally added components. Results: The detection limits of 9 sweeteners were 2.5 ~ 20μg · mL-1, 5 samples of maltitol, isomaltulose, D-sorbitol and D-mannitol were detected, and 9 kinds of sweet taste Secondary mass spectrometry lysis pathway. Conclusion: The method is simple and rapid, the detection time is only a few seconds, and the detection efficiency is nearly 25 times higher than that of liquid chromatography. It is suitable for rapid screening of sweeteners in large quantities of wine.