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目的:研究补肾法对肾虚排卵抑制模型大鼠内分泌激素、脏器湿重及抑制素的影响。方法:采用羟基脲灌胃复制肾虚排卵抑制大鼠模型,造模成功后予以罗氏促排卵汤和/或针灸补肾法治疗,治疗后观察大鼠内分泌激素、脏器湿重及抑制素的变化。结果:羟基脲造模可以使模型大鼠出现肾虚兼脾虚表现,卵巢病理切片提示排卵抑制;针刺加中药治疗后大鼠肾虚兼脾虚症状改善,内分泌激素及脏器湿重均有不同程度的增加,治疗后大鼠体重明显增加,子宫、卵巢湿重增加,差异有显著性意义(P<0.05);内分泌指标、抑制素无显著性差异(P>0.05)。结论:补肾法(包括补肾复方和/或针刺加电针)对肾虚-排卵抑制病理模型大鼠进行治疗,可以有效增加子宫及卵巢湿重,但对大鼠其它脏器湿重、内分泌及抑制素指标无明显影响。
Objective: To study the effect of tonifying kidney on endocrine hormones, organ weight and inhibin in rats with kidney deficiency ovulation inhibition. Methods: The rat model of renal ovulation suppression was established by intragastric administration of hydroxyurea. The rats were treated with Roche-Ovulation Decoction and / or acupuncture and tonifying kidney method after the model was established. After treatment, the change of endocrine hormones, organ weight and inhibin were observed. Results: Hydroxyurea modeling could make kidney and spleen deficiency appear in model rats, and ovarian pathology showed ovulation inhibition. After acupuncture plus traditional Chinese medicine treatment, symptoms of kidney and spleen deficiency in rats were improved, endocrine hormones and organ weight were all different (P <0.05). There was no significant difference between endocrine and inhibin (P> 0.05). Conclusion: Bushen method (including tonifying kidney and / or acupuncture plus electroacupuncture) can improve uterine and ovarian wet weight in rats with renal deficiency and ovulation suppression, but have no effect on wet weight and endocrine Inhibin index no significant effect.