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目的确定长柱金丝桃中是否含有金丝桃素及金丝桃素含量的变化规律。方法使用体积分数80%的乙醇进行提取,利用高效液相色谱仪进行金丝桃素的检测。结果长柱金丝桃中含有金丝桃素;金丝桃素的含量随季节变化,茎、叶5月份较高,以后下降,8月最低;花的变化与季节变化关系不大;同一时期不同器官含量不同,其中花中含量最高,叶中含量居中,茎中含量最低。结论长柱金丝桃中含有金丝桃素,金丝桃素含量随着季节和器官的不同而变化。
Objective To determine if hypericin and hypericin content in the long-column hypericum contained changes. Methods The volume fraction of 80% ethanol was used for extraction, using high performance liquid chromatography for the detection of hypericin. Results Hypericin contained hypericin. The content of hypericin varied with the seasons. The content of hypericin in stems and leaves was higher in May and later decreased in August, while the change in flowers had little to do with the seasonal changes. In the same period Different organs have different contents, among which the content of flowers is the highest, the content of leaves is middle and the content of stems is the lowest. Conclusion Hypericin contains hypericin. Hypericin content varies with season and organ.