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为明确玉米对纹枯病的抗病机制,以玉米自交系CML429(抗)×DM9(感)的182个F2单株为作图群体,构建了包含82个SSR标记位点的遗传连锁图谱,覆盖玉米基因组的1530.9cM,标记间平均图距为18.67cM。通过人工接种分析F2:3群体对纹枯病病菌的抗性表现,用复合区间作图法分析抗病QTL及遗传效应,共检测到4个抗性QTLs,分布于第6、7和10条染色体上,在第6染色体上检测到2个QTLs,分别与标记bnlg107、umc1796连锁,其遗传效应分别能解释表型方差的12.63%和0.27%;在第7、10染色体上各检测出1个QTL,分别与标记bnlg1161、phi059连锁,其遗传效应能分别能解释表型方差的15.21%和5.42%。
In order to clarify the mechanism of resistance to sheath blight in maize, 182 F2 individuals of inbred lines CML429 (resistant) × DM9 (susceptible) were used as a mapping population to construct a genetic linkage map containing 82 SSR markers , Covering 1530.9cM of maize genome, the average distance between markers was 18.67cM. The resistance of Rhizoctonia solani to F2: 3 was analyzed by artificial inoculation, and QTLs and genetic effects were analyzed by composite interval mapping. A total of 4 QTLs were detected and distributed in 6, 7 and 10 On chromosome 6, two QTLs were detected on chromosome 6, which were linked with markers bnlg107 and umc1796, respectively. The genetic effects explained 12.63% and 0.27% of the phenotypic variance, respectively; one on chromosomes 7 and 10 The QTLs were linked to the markers bnlg1161 and phi059, respectively. The genetic effects explained 15.21% and 5.42% of the phenotypic variance, respectively.