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目的 :探讨nm2 3基因表达在原发性肺鳞癌中的作用。方法 :采用nm2 3基因寡核酸探针 ,通过Northern印迹转移的方法 ,检测 17例原发性肺鳞癌患者手术切除的癌组织 ,癌旁组织和远离癌灶的非癌肺组织中nm2 3基因mRNA表达水平 ,并分析nm2 3mRNA表达水平与肺癌生物特性的相关性。结果 :肺鳞组织nm2 3基因mRNA表达水平增高 (8.17± 3.41)与癌旁组织 (4 .0 5± 1.90 )及远离癌灶的非癌肺组织 (3 .2 1± 1.97)比较差异有显著性 (P <0 .0 5 )。有淋巴结转移者nm2 3基因mRNA表达水平 (10 .5 4± 3.6 0 )与无淋巴结转移者(5 .72± 2 .5 1)差异有显著性 (P <0 .0 1) ;癌细胞低分化者 (9.83± 3.2 7)与中高分化者 (5 .96± 2 .6 3)差异亦有显著性 (P <0 .0 2 )。结论 :肺鳞癌nm2 3基因mRNA表达水平增高 ,且与癌的淋巴转移和组织分化有关 ,但未显示nm2 3基因mRNA表达在肺鳞癌中的癌转移抑制作用
Objective: To investigate the role of nm23 gene in primary lung squamous cell carcinoma. Methods: The nm23 gene of 17 cases of primary lung squamous cell carcinoma was examined by Northern blotting with nm2 3 gene oligonucleotide probe. mRNA expression levels, and analysis of nm23mRNA expression level and the biological characteristics of lung cancer. Results: The mRNA expression of nm23 gene in lung squamous tissue increased (8.17 ± 3.41) compared with the adjacent tissue (4. 05 ± 1.90) and non-cancerous lung tissue (3.21 ± 1.97) Sex (P <0.05). The level of nm23 gene mRNA in lymph node metastasis was significantly higher than that in non-lymph node metastasis (10.4 ± 3.6 0) (5.72 ± 2.51) (P <0.01) Differences (9.83 ± 3.2 7) and moderately well-differentiated (5.96 ± 2.63) were also significantly different (P <0. CONCLUSION: The mRNA expression of nm23 gene in squamous cell carcinoma of the lung is increased, which is related to the lymphatic metastasis and tissue differentiation of lung squamous cell carcinoma. However, the inhibition of the metastasis of nm23 gene in lung squamous cell carcinoma is not demonstrated