论文部分内容阅读
以15份葡萄品种为试材,采用SSR分子标记技术,研究了15份葡萄品种资源的遗传多样性。结果表明:从245对引物中筛选出49对清晰、稳定性好的多态性引物。49对引物在15份材料中共扩增出235条带,均为多态性条带,多态性百分率为100%。每对引物扩增的等位位点数为2~9个,相似性系数分析结果表明,供试的15份葡萄材料的遗传相似系数为0.660~0.860,其平均遗传相似系数为0.760。聚类分析表明,在遗传相似系数0.672处,15份葡萄材料可分为三大类群。第一大类包括2份欧美杂种(V.vinifera L.×V.labrusca L.)和1份美洲种(V.labrusca L.)品种;第二大类为8份欧亚种(V.vinifera L.)葡萄品种;第三大类包括2份山欧杂种(V.amurensis Rupr.×V.viniferaL.)和2份山葡萄(V.amurensis Rupr.)品种。可见,欧亚种和美洲种葡萄亲缘关系相对较近,而山葡萄与二者亲缘关系相对较远。这些引物中,VMCNG4B9、VMC3G9能将15个葡萄品种区分开,为今后开展葡萄品种指纹图谱构建奠定基础。
Fifteen grape cultivars were used as materials to study the genetic diversity of 15 grape cultivars using SSR markers. The results showed that: 49 pairs of 245 pairs of primers were screened for clear and stable polymorphic primers. A total of 235 bands were amplified by 49 pairs of primers from 15 materials, all of which were polymorphic bands with 100% polymorphism. The number of alleles per primer pair was 2 to 9. The similarity coefficient analysis showed that the genetic similarity coefficients of 15 grape materials tested ranged from 0.660 to 0.860, and the average genetic similarity coefficient was 0.760. Cluster analysis showed that at the genetic similarity coefficient of 0.672, 15 grape materials could be divided into three groups. The first category consists of two species of V. vinifera L. × V. labrusca L. and one species of V. labrusca L. The second group consists of eight species of V. vinifera L.) grape varieties; the third largest category includes 2 varieties of V. amurensis Rupr. × V.vinifera L. and 2 parts of V. amurensis Rupr. Can be seen, Eurasian species and American grape relatives are relatively close, while the grape and the two relatively distant genetic relationship. Among these primers, VMCNG4B9 and VMC3G9 could distinguish 15 grape cultivars, which laid the foundation for the future development of grape variety fingerprinting.