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本文制备了一种以溶剂聚合膜ｐＨ电极作原电极，经高碘酸钠话化的醋酸纤维膜固定乙酰胆碱酯酶的乙酰胆碱生物电极。研究了酶固定化条件的影响，测得静态和动力学条件下电极的线性响应范围分别为２４０～１３００μｇ／ｍＬ和３００～１７００μｇ／ｍＬ，将电极用于实际样品的回收率试验中，获得了满意的结果。
This paper prepared a solvent polymerization membrane pH electrode as the original electrode, the sodium periodate sodium acetate membrane fixed acetylcholinesterase acetylcholine bioelectrode. The influence of enzyme immobilization conditions was studied. The linear response range of the electrode was 240 ~ 1300μg / mL and 300 ~ 1700μg / mL respectively under static and dynamic conditions. The electrode was used in the recovery of the actual samples, Satisfying result.