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目的探讨高原低氧对大鼠血清中低氧诱导因子-1α(HIF-1α)含量和血浆中活性氧(ROS)含量及肝细胞超微结构的影响。方法将50只健康成年SPF级雄性SD大鼠随机分为对照组(海拔2 262 km)和高原组(海拔4 300km),每组25只。喂养30 d后,测定血清HIF-1α和血浆ROS水平,观察肝细胞超微结构。结果与对照组比较,高原组大鼠血清中HIF-1α含量明显增高,血浆中ROS含量明显降低,差异均有统计学意义(P<0.01);肝细胞线粒体肿胀,粗面内质网扩张,部分轻度脱粒现象。结论高原低氧环境可能通过调节ROS、HIF-1α水平保护细胞,促进低氧适应。
Objective To investigate the effects of plateau hypoxia on the levels of hypoxia inducible factor-1α (HIF-1α) and reactive oxygen species (ROS) in plasma and the ultrastructure of hepatocytes in rats. Methods Fifty healthy adult Sprague-Dawley (SD) male SD rats were randomly divided into control group (2 262 km above sea level) and plateau group (4 300 km above sea level) with 25 rats in each group. After feeding for 30 days, the levels of serum HIF-1α and plasma ROS were measured to observe the ultrastructure of hepatocytes. Results Compared with the control group, the level of HIF-1α in the plateau group was significantly increased and the level of ROS in the plasma was significantly decreased (P <0.01). The hepatocyte mitochondria swelling, rough endoplasmic reticulum dilation, Partially mild threshing phenomenon. Conclusion The plateau hypoxic environment may protect the cells by regulating the levels of ROS and HIF-1α, and promote hypoxia adaptation.