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为了研究甲状旁腺素（ｐａｒａｔｈｙｒｏｉｄｈｏｒｍｏｎｅ，ＰＴＨ）促成骨样细胞ＲＯＳ１７／２．８增殖分化的信号传递的机理，观察了ＰＴＨ对细胞内酪氨酸蛋白激酶（ｔｙｒｏｓｉｎｅｐｒｏｔｅｉｎｋｉｎａｓｅｓ，ＴＰＫ）活性及酪氨酸蛋白磷酸化水平的影响，并检测了ＰＴＨ对ｃ－ｒａｓｍＲＮＡ表达的诱导作用．结果表明，ＰＴＨ可增强细胞内ＴＰＫ活性，提高细胞内酪氨酸蛋白磷酸化水平，以及促进ｃ－ｒａｓｍＲＮＡ的持续表达．这些结果提示，ＰＴＨ促增殖的细胞内信息传递通路与酪氨酸蛋白激酶－ｒａｓ－ＭＡＰＫｉｎａｓｅ通路密切相关，而ＰＫＡ／ＰＫＣ通路的最终效应可能是通过与上述通路的ｃｒｏｓｓｔａｌｋ实现的
In order to study the mechanism of parathyroidhormone (PTH) promoting proliferation and differentiation of osteoblast-like cells ROS17 / 2.8, the effects of PTH on tyrosine protein kinase (TPK) activity and tyrosine protein Phosphorylation, and examined the induction of c-rasmRNA expression by PTH. The results showed that PTH can enhance intracellular TPK activity, increase intracellular tyrosine protein phosphorylation, and promote c-rasmRNA sustained expression. These results suggest that the intracellular signaling pathway that PTH promotes proliferation is closely related to the tyrosine kinase-Ras-MAPKinase pathway, and the ultimate effect of the PKA / PKC pathway may be through crosstalk with the above pathway