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目的观察CD99在部分小细胞恶性肿瘤中的表达及意义。方法对257例骨和软组织小细胞恶性肿瘤采用EnVision二步法检测CD99的表达。结果257例中CD99在间叶性软骨肉瘤的阳性率为93.3%(14/15),在骨内EWS及软组织内EWS/PNET的阳性率为100%(97/97);在浆细胞骨髓瘤的阳性率为2.7%(2/73);在骨内原发性恶性淋巴瘤(均为B细胞型非霍奇金淋巴瘤)的阳性率为13.5%(5/37);在小细胞型骨肉瘤的阳性率为95%(17/18);在骨转移性小细胞癌的阳性率为70%(7/10);在以梭形细胞为主的单向型滑膜肉瘤的阳性率为100%(5/5);1例促结缔组织增生性小细胞肿瘤为(+);而1例软组织胚胎性横纹肌肉瘤为(-)。结论CD99可在多种小细胞恶性肿瘤中有阳性表达。此抗体在骨及软组织小细胞恶性肿瘤的鉴别诊断中特异性较差,CD99单项阳性不能作为鉴别诊断依据。
Objective To observe the expression and significance of CD99 in some small cell malignant tumors. Methods The EnVision two-step method was used to detect the expression of CD99 in 257 small-cell malignant tumors of bone and soft tissue. Results The positive rate of CD99 in mesenchymal chondrosarcoma was 93.3% (14/15) in 257 cases, and the positive rate of EWS/PNET in bone in EWS and soft tissue was 100% (97/97); in plasma cell myeloma The positive rate was 2.7% (2/73); the positive rate of primary malignant lymphoma in bone (all B-cell non-Hodgkin’s lymphoma) was 13.5% (5/37); in small cell type The positive rate of osteosarcoma is 95% (17/18); the positive rate in small metastatic bone cell carcinoma is 70% (7/10); the positive rate of unidirectional synovial sarcoma, mainly spindle cells 100% (5/5); 1 case of connective tissue proliferating small cell tumors (+); and 1 case of soft tissue embryonal rhabdomyosarcoma (-). Conclusion CD99 can be positively expressed in a variety of small cell malignant tumors. This antibody has poor specificity in differential diagnosis of bone and soft-tissue small-cell malignancies, and CD99 single-positive can not be used as the basis for differential diagnosis.