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目的分离并纯化毛花猕猴桃根中活性成分2α,3α,24-三羟基-12-烯-28-乌苏酸,建立HPLC测定毛花猕猴桃根中2α,3α,24-三羟基-12-烯-28-乌苏酸含量的方法,并比较不同提取部位样品中及不同产地的毛花猕猴桃根中该活性成分的含量差异。方法利用HPLC-PDA色谱分析法进行含量测定方法学考察,并以标准曲线法对5个不同来源批次的毛花猕猴桃根提取物及不同提取部位中2α,3α,24-三羟基-12-烯-28-乌苏酸的含量进行测定。结果福州寿宁县及丽水云和县产毛花猕猴桃根中2α,3α,24-三羟基-12-烯-28-乌苏酸含量较高,而在温州永嘉县及丽水市产样品中含量较低。且二氯甲烷提取部位含量最高,乙醇部位次之,甲醇部位最低。结论该测定方法精确稳定,重复性好,可作为毛花猕猴桃根中活性成分的定量测定方法。不同产地的毛花猕猴桃根中2α,3α,24-三羟基-12-烯-28-乌苏酸的含量存在差异,且二氯甲烷提取部位含量最高。
OBJECTIVE To isolate and purify the active ingredients 2α, 3α, 24-trihydroxy-12-en-28-ursuic acid from the root of Actinidia chinensis and establish HPLC determination of 2α, 3α, 24-trihydroxy-12- -28-ursolic acid content of the method, and compare the different parts of the sample extract and different regions of the kiwifruit root content difference in the active ingredient. Methods HPLC-PDA chromatographic analysis was used to determine the content of 2α, 3α, 24-trihydroxy-12-β-D-galactosidase in different extracts of kiwifruit from five different batches. Ene-28-ursolic acid content was measured. Results The content of 2α, 3α, 24-trihydroxy-12-ene-28-ursuic acid in the root of kiwifruit from Shouning County and Lishui County in Fuzhou was higher than those in Yongjia County and Lishui City in Wenzhou City low. And the highest content of dichloromethane extraction parts, followed by the ethanol part, the lowest methanol parts. Conclusion The method is accurate and reproducible, and can be used as a quantitative method for the determination of active components in the root of Actinidia. The contents of 2α, 3α, 24-trihydroxy-12-en-28-ursuic acid in the kiwifruit root of different regions were different, and the content of methylene chloride was the highest.