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采用RT-PCR方法从葡萄(Vitis vinifera)叶片中克隆到1个ACC氧化酶(ACC Oxidase,ACO)基因,命名为VvACO1。序列分析表明,VvACO1的开放阅读框长度为957 bp,编码318个氨基酸,具有亮氨酸拉链、Fe~(2+)结合基序和抗坏血酸结合位点等ACO蛋白的典型结构域;与烟草和番茄的ACO蛋白的相似度分别为87%和84%。组织特异性及乙烯诱导表达结果表明,VvACO1在葡萄叶片、卷须、幼茎、果实、花序、须根以及腋芽等组织中差异表达,并受到乙烯利的诱导上调表达。过表达VvACO1的番茄开花期及果实成熟期提前,过表达VvACO1的拟南芥叶片光合效率和叶绿素a的荧光强度明显下降。
One ACC oxidase (ACO) gene was cloned from the leaves of Vitis vinifera by RT-PCR and named as VvACO1. Sequence analysis showed that the open reading frame of VvACO1 was 957 bp, encoding a protein of 318 amino acids. It contained typical domains of ACO such as leucine zipper, Fe ~ (2+) binding motif and ascorbic acid binding site. The similarity of tomato ACO protein was 87% and 84%, respectively. Tissue-specific and ethylene induced expression results showed that VvACO1 was differentially expressed in grapevine leaves, tendrils, young stems, fruits, inflorescences, fibrous roots and axillary buds, and up-regulated by ethephon. The VvACO1-overexpressed tomato had earlier flowering and fruit ripening stage, and the photosynthetic efficiency and chlorophyll a fluorescence intensity of Arabidopsis over-expressed VvACO1 significantly decreased.