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采用ＰＣＲ和寡核苷酸探针杂交技术检测了３５例结直肠癌和５例息肉活检组织ｋｉ—ｒａｓ基因第１２位密码子的点突变。发现１０例结直肠癌具有点突变，其中ＧＧＴ→ＧＴＴ（Ｇｌｙ→Ｖａｌ）突变３例，ＧＧＴ→ＧＡＴ（Ｇｌｙ→Ａｓｐ）突变７例；１例息肉有突变（ＧＧＴ→ＧＴＴ）。
The point mutation of the 12th codon of the ki-ras gene in 35 cases of colorectal cancer and 5 cases of polyp biopsy was detected by PCR and oligonucleotide probe hybridization. 10 cases of colorectal cancer were found to have point mutations, of which GGT→GTT (Gly→Val) mutations were 3 cases, GGT→GAT (Gly→Asp) mutations were 7 cases, and 1 case of polyps had mutations (GGT→GTT).