[Objectives]As a traditional inoculation method of rice sheath blight,rice relative lesion height method needs rice grow to late tillering or heading stage and then inoculate in vivo,which is time-consuming and is difficult to control the testing conditions,so it is urgent to search a better alternative method.[Methods]With rice relative lesion height method( in vivo inoculation) as the control,the pathogenicity and crude toxin activity of Rhizoctonia solani were determined by rice detached leaf sheath method and rice detached leaf method,to compare the correlation of in vivo and in vitro inoculation. [Results] Rice detached leaf method and rice detached leaf sheath method had significantly positive correlation with rice relative lesion height method,and the determination results of rice detached leaf method and rice detached leaf sheath method could reflect the pathogenicity and crude toxin activity of R. solani accurately. [Conclusions]Rice detached leaf method and rice detached leaf sheath method are easy to operate and control testing conditions,and could be used as alternative methods to determine pathogenicity and crude toxin activity of R. solani. [Objectives] As a traditional inoculation method of rice sheath blight, rice relative lesion height method needs rice grow to late tillering or heading stage and then inoculate in vivo, which is time-consuming and is difficult to control the testing conditions, so it is urgent to search a better alternative method. [Methods] With rice relative lesion height method (in vivo inoculation) as the control, the pathogenicity and crude toxin activity of Rhizoctonia solani were determined by rice detached leaf sheath method and rice detached leaf method, to compare the correlation of in vivo and in vitro in. [Results] Rice detached leaf method and rice detached leaf sheath method had significantly positive correlation with rice relative lesion height method, and the determination results of rice detached leaf method and rice detached leaf sheath method could reflect the pathogenicity and crude toxin activity of R. solani accurately. [Conclusions] Rice detached leaf method and rice detached leaf sheath method are easy to operate and control testing conditions, and could be used as alternative methods to determine pathogenicity and crude toxin activity of R. solani.