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利用20个随机引物对产于方正、彭泽和淇河地区的三个银鲫种群进行了RAPD检测,结果显示14个引物的扩增效果良好,单一引物扩增的DNA片段数在2-13条之间,片段长度在300-4000bp之间。根据RAPD结果,进行了银鲫种群内、种群间的遗传差异分析。14个引物中,引物OPV-07、OPV-14和OPV-20等对三种群银鲫扩增出的指纹图谱差异显著,可作为银鲫种群鉴定的分子标记。
RAPD analysis of three silver crucian carp populations produced in Founder, Pengze and Qihe regions using 20 random primers showed that the amplification results of 14 primers were good, and the number of DNA fragments amplified by single primer ranged from 2 to 13 Between the fragments, the fragment length between 300-4000bp. According to the results of RAPD, genetic differences among populations within and among populations of silver crucian carp were analyzed. Fourteen primers, primer OPV-07, OPV-14 and OPV-20 and other three groups of silver carp amplified fingerprints significant differences, can be used as silver carp populations identified molecular markers.