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本文报道茄属果树可乐茄（ＳｏｌａｎｕｍｑｕｉｔｏｅｎｓｅＬａｍ．）叶肉原生质体的分离、培养及植株再生。幼嫩叶片原生质体经酶游离、纯化后，以１×１０４个／ｍｌ密度培养于稍加改良Ｋ８ｐ（附加２，４－Ｄ０．５ｍｇＬ（－１）、ＮＡＡ１．０ｍｇＬ（－１）和ＢＡ０．５ｍｇＬ（－１））的培养基中，三天后开始分裂，一周分裂３—４次。一个月形成小细胞团，植板率为０．１—０．２％，小细胞团转培养于ＭＳ＋２，４－Ｄ０．５ｍｇＬ（－１）上增殖后进行分化。原生质体来源愈伤组织在ＩＡＡ（０．１—１．０ｍｇＬ（－１））与ＢＡ或ＺＴ组合的培养基中能诱导器官发生，芽分化率最高可达４２．９％；但ＩＡＡ、ＢＡ、ＺＴ三者一起使用未见任何器官分化。小芽在ＭＳ＋ＩＡＡ０．２ｍｇＬ（－１）中生根成植株。可乐茄叶肉原生质体的植株再生，可应用于育种和茄属植物遗传工程研究。
In this paper, the isolation, culture and plant regeneration of solanum mesophyll from Solanum citriense Lam. The young leaf protoplasts were enzymatically dissociated and purified. The purified protoplasts were cultured in slightly modified K8p (supplemented with 2,4-D0.5mgL (-1), NAA1.0mgL (-1)) and BA0 at a density of 1 × 104 / ml. 5mgL (-1)) medium, dividing began three days later, dividing 3-4 times a week. One month to form small cell mass, the plating rate of 0.1-0.2%, small cell mass transfer culture MS + 2,4-D0.5mgL (-1) proliferation after differentiation. Protoplast-derived callus induced organogenesis in IAA (0.1-1.0mgL (-1)) and BA or ZT medium with the highest rate of shoot differentiation up to 42.9%. However, IAA, BA , ZT three together without any organ differentiation. Small shoots were rooted in MS + IAA 0.2 mg L (-1). Plant regeneration of Coleoptera mesophyll protoplasts can be applied in breeding and genetic engineering of Solanum.