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目的观察弓形虫感染对大鼠海马超微结构、海马组织超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量以及空间学习记忆能力的影响。方法将20只成年雄性SD大鼠随机分成2组:弓形虫感染组和健康对照组。弓形虫感染组每只大鼠腹腔注射纯化弓形虫速殖子107/ml×2 ml;健康对照组大鼠每只腹腔注射2 ml灭菌生理盐水。弓形虫感染9周后Morris水迷宫法测试各组大鼠空间学习记忆能力;应用黄嘌呤氧化酶法测定SOD活性;硫代巴比妥酸比色法测定MDA含量;透射电镜观察大鼠海马线粒体和突触结构的改变。结果 Morris水迷宫平均潜伏期弓形虫感染组大鼠(26.07±10.08)s,较对照组(13.46±5.43)s明显延长(P<0.05);弓形虫感染组大鼠海马SOD活性(49.74±4.44)NUI/mgprot,较对照组(58.19±6.83)NUI/mgprot降低(P<0.05);海马MDA含量感染组(4.48±0.65)nmol/mg-prot,较对照组(3.51±0.53)nmol/mgprot升高(P<0.05)。透射电镜观察弓形虫感染组大鼠海马细胞线粒体稍有扩张,粗面内质网、核糖体依然可见,但稍欠丰富;核膜基本清晰,无染色质聚集;线粒体膜尚基本完整,髓鞘轻度崩解,游离核蛋白体多。结论弓形虫感染可使大鼠海马自由基清除能力下降,氧化应激反应增强。
Objective To investigate the effects of Toxoplasma gondii infection on the ultrastructure of hippocampus, the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in the hippocampus and spatial learning and memory of rats. Methods Twenty adult male Sprague-Dawley rats were randomly divided into two groups: Toxoplasma gondii infection group and healthy control group. Toxoplasma gondii infection group were injected intraperitoneally with Toxoplasma gondii 107 / ml × 2 ml; healthy control rats were injected intraperitoneally with 2 ml sterile saline. Nine weeks after Toxoplasma gondii infection, the spatial learning and memory abilities of rats in each group were measured by Morris water maze test, the SOD activity was measured by xanthine oxidase method, the content of MDA was detected by thiobarbituric acid colorimetric method, the mitochondria And changes in synaptic structure. Results The mean latency of Toxoplasma gondii infection group (26.07 ± 10.08 s) was significantly longer than that of control group (13.46 ± 5.43 s) (P <0.05), and the activity of SOD in hippocampus of Toxoplasma gondii infection group was 49.74 ± 4.44 NUI / mgprot was significantly lower than that of the control group (58.19 ± 6.83) (P <0.05). The content of MDA in hippocampus was (4.48 ± 0.65) nmol / mg-prot, High (P <0.05). Transmission electron microscopy showed that the mitochondria of hippocampus in Toxoplasma gondii infection group were slightly expanded, while the rough endoplasmic reticulum and ribosomes were still visible but slightly rich. The nuclear membrane was clear and chromatin-free. The mitochondrial membrane was still intact, myelin Slight disintegration, free ribosomes and more. Conclusion Toxoplasma gondii infection can reduce the scavenging capacity of free radicals in hippocampus of rats and increase the oxidative stress response.