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我们用制备几个肝癌细胞系的单克隆抗体研究并证实与变异肝细胞有关的一些表面抗原。用免疫过氧化酶染色方法对肝细胞瘤、正常组织和其他恶性肿瘤进行比较,部分地证实这些抗体对存在于肝细胞瘤上的抗原决定簇的特异性。由单克隆抗体识别的抗原已经从清洁剂处理过的细胞中得到免疫沉淀。其分子量与SDS聚丙烯胺凝胶电泳法所得的结果接近。使人感到兴趣的是,有些抗体能识别来自肝活检和手术切除的人体肝癌组织存在的抗原决定簇。我们用~(131)I标记抗体和核扫描在裸鼠中生长的人体肝癌实验中,表明了单克隆抗体是肿瘤定位的有潜在力的试剂。
We used monoclonal antibodies to prepare several liver cancer cell lines to study and confirm some of the surface antigens associated with the mutant liver cells. Comparison of hepatomas, normal tissues, and other malignancies using immunoperoxidase staining methods partially confirms the specificity of these antibodies for antigenic determinants present on hepatomas. Antigens recognized by monoclonal antibodies have been immunoprecipitated from detergent-treated cells. Its molecular weight is similar to that obtained by SDS polyacrylamide gel electrophoresis. Interestingly, some antibodies recognize antigenic determinants in human liver cancer tissue from liver biopsy and surgical resection. We used ~(131)I-labeled antibodies and nuclear scans in human liver cancer experiments grown in nude mice, indicating that monoclonal antibodies are potentially powerful agents for tumor localization.