【摘 要】
：
Ubiquitin-specific protease 31 (USP31) is a member of deubiquitinase family that is involved in nuclear factor-κB activation and sarcomagenesis.However,little is known about posttranslational modification in the regulation of its activity and cervical can
【机 构】
：
Hubei Key Laboratory of Edible Wild Plants Conservation and Utilization,Hubei Engineering Research C
论文部分内容阅读
Ubiquitin-specific protease 31 (USP31) is a member of deubiquitinase family that is involved in nuclear factor-κB activation and sarcomagenesis.However,little is known about posttranslational modification in the regulation of its activity and cervical cancer cell growth.In our study,we found that the Lys1264 residue of USP31 can be modified with an acetyl group by high-resolution mass spectrometry in HeLa cell line,and site-specific mutagenesis can significantly increase USP31 ubiquitin hydrolase activity and decrease the expression of p65.When being transfected with a plasmid expressing mutated USP31,the number of cancer cells was significantly decreased.We also observed that mutated USP31 could promote apoptosis but not cell cycle by flow cytometer analysis.Overexpression of mutated USP31 could reverse the effect in USP31 knockdown cell line.To further investigate its activity in tumorigenesis,deacetylase sirtuin 1 (Sirt1) was shown to inter-act with USP31 by co-immunoprecipitation and blocking the function of Sirt1 by knockdown or the inhibitor nicotinamide could increase the acetylation of USP31.When Lys1264 of USP31 mutated,Sirt1 could not remove its acetylation and alter the expression level of p65.Finally,inhibition or knockdown of Sirt1 suppressed USP31 activity in HeLa cell line,leading to cisplatin-induced apop-tosis resistance.Therefore,acetylation at Lys1264 suppresses USP31 activity and plays a protective role in cancer cell growth.Our study contributes to understanding the mechanism of USP31 activity regulation and its role in tumorigenesis.
其他文献
今年1～6月,我国制造业投资同比增长19.2％,高于全部投资6.6个百分点,高技术制造业投资增长29.7％,高于全部投资12.7个百分点.这些数据表明,制造业投资正在回暖.rn制造业内生增长动力有所减弱rn制造业是实体经济的根基.当前,我国正在由制造大国向制造强国迈进.2020年,我国制造业增加值达到3.85万亿美元,大致相当于美国、日本、德国制造业增加值之和;我国制造业增长值全球占比超过28％,对全球制造业增长的贡献超过35％.建设制造强国已成为我国实现高质量发展、构建新发展格局的重要抓手.
Fibroblast growth factor-binding protein 1 (FGFBP1) promotes fibroblast growth factor (FGF) activ-ity by releasing FGFs from extracellular matrix storage.We previously reported that the tumor suppressor F-box and WD repeat domain-containing 7 suppresses F
今年已90岁高龄的全国劳模、“七一勋章”获得者黄宝妹,一辈子都住在上海杨浦滨江.rn曾在国棉十七厂工作大半辈子的她,见证了江边巨变.昔日“工业锈带”转型“生活秀带”,一条“生产之江”成为人人可共享的“生活之江”.rn习近平总书记“七一”重要讲话强调“坚持全心全意为人民服务的根本宗旨,站稳人民立场”.上海杨浦区委书记谢坚钢说,在杨浦滨江的建设、改造中,我们始终从群众切身感受出发,聚焦群众需求,坚持以人民为中心,打造人民城市建设的示范区.
他是新中国体育众多成就的缔造者,培养出我国第一批体育专业学生,建立起我国最早的青训体系,率先创办了新中国第一个体育科学研究所,开创了体育教育事业的先河.rn他是我国体育教育事业的坚强脊梁,涌现出以宋君复、徐英超、李鹤鼎等“九大教授”为代表的名家名师,造就了多项体育运动先驱,奠定了新中国体育学科发展之基.
“数字应用第一省”建设提速快跑、数字经济点亮高质量发展强劲引擎,高能级创新平台打造战略科技力量,“海上福建”建设乘风破浪、精彩纷呈,山海协作弥合区域发展落差,经济“高素质”与生态“高颜值”相得益彰……rn潮涌东南、扬帆八闽.作为21世纪海上丝绸之路核心区、国家生态文明试验区,当下福建省正立足特色优势,统筹做好数字经济、海洋经济和绿色经济,加快培育经济新增长点,努力建设“机制活、产业优、百姓富、生态美”的新福建,奋力谱写全面建设社会主义现代化国家的福建篇章.
一根牛毛能做什么?陕西微雕匠人卢彦卓有自己的答案——把古诗刻在上面.rn在直径以毫米计的空间里,他探索微雕工艺的极限;在小小的一块材料上,以刀代笔,书写人生与追求.rn来到微雕的世界rn2018年,卢彦卓开始接触微雕.rn经人介绍,他去杭州拜访了一位微雕大师,二人一起前往微雕博物馆,那里收集着全球各地微雕艺术家的作品.第一次看到微雕,卢彦卓就被深深地震撼了:“感觉像是进入了另一个世界.”
“我们实现了第一个百年奋斗目标,在中华大地上全面建成了小康社会”.这一庄严宣告标志着,中国历史性地解决了绝对贫困问题.中国共产党带领中国人民历经百年奋斗,终使“民亦劳止,汔可小康”的千年梦想照进现实.
Propofol is the most commonly used intravenous anesthetic worldwide.It can induce loss of consciousness prior to the occurrence of severe respiratory suppression,which is also a phar-macodynamic feature of all general anesthetics.However,the neural mechan
你以为的“种草笔记”,不过是团队操作的虚假“安利”——近日,有业内人士起底,在利益驱动下,伪造素人“种草笔记”的灰色产业链已渗透至各大UGC(用户原创内容)平台.rn代写代发所谓的原创内容是否违法?如何防范、监管利用虚假笔记引流、带货?以营利为目的的“种草笔记”,如何完成利益收割?
上半年固定资产投资完成额同比增长15.2％,高于全国平均水平2.6个百分点,比2019年上半年增长15.6％,两年平均增长7.5％,高于全国平均水平3.1个百分点,同比增速居全国第10位,比一季度提升10个位次.这是今年上半年黑龙江省交出的亮眼“答卷”.rn数据背后,是黑龙江省以“钢牙啃硬骨头”猛药治沉疴重塑营商环境的系列举措.从“办事不求人”的“小切口”入手,到构建“极简审批”模式,破除市场主体投资兴业的壁垒,再到对标对表先进城市发展理念和经验做法,针对自身差距,梳理、审视、集合各项发展措施,黑龙江营商