论文部分内容阅读
白杄(Picea meyeri Rehd.et Wils.)的成熟胚在4～6℃低温下保存1个月后,接种于改良LP+2mg/L 2,4-D+1mg/L 6-BA的培养基上,黑暗条件下培养1个月便可产生白色半透明的胚性愈伤组织。整体染色封片观察表明,胚性愈伤组织由很多很长的胚柄细胞及其顶端的胚细胞团组成,这种愈伤组织培养物称为胚性胚柄团。胚性胚柄团在MS+1mg/L 2,4-D+1mg/L KT的继代培养基上黑暗条件下可保持旺盛的增殖能力和分化潜力。当胚性胚柄团转到MS+5mg/L ABA+5mg/L AgNO_3的分化培养基上,1个月后可产生大量正常的体细胞胚。体细胞胚成熟以后转到含0.5％活性炭的无激素1/2MS基本培养基上约40d后可长出1.5～2.5cm的根,约60d后可长出真叶。光、ABA、蔗糖及AgNO_3浓度是影响体细胞胚发生的主要因素。
Mature embryos of Picea meyeri Rehd. Et Wils. Were kept for 1 month at 4 ~ 6 ℃ low temperature and then inoculated into medium of modified LP + 2mg / L 2,4-D + 1mg / L 6-BA On the dark conditions for 1 month can produce white translucent embryogenic callus. The whole staining showed that the embryogenic calluses consisted of many long embryoid cells and the apical cell clusters at the top. This callus culture was called the embryogenic chorion mass. Embryogenic chondrocytes maintained vigorous proliferative and differentiation potential under dark conditions on MS + 1 mg / L 2,4-D + 1 mg / L KT subculture medium. When embryogenic hodggs were transferred to MS + 5mg / L ABA + 5mg / L AgNO_3 differentiation medium, a large number of normal somatic embryos could be produced after one month. Somatic embryos after maturity transferred to 0.5% activated carbon hormone-free 1 / 2MS basic medium about 40d after the growth of 1.5 ~ 2.5cm roots, about 60d after the growth of true leaves. Light, ABA, sucrose and AgNO 3 concentrations were the main factors affecting somatic embryogenesis.