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以含有160个株系的四向重组自交系为试验材料,通过188个SSR标记鉴定个体基因型,利用单标记分析方法,分析了2年4点试验获得全生育期表型数据,对该群体的全生育期进行了QTL定位分析。结果表明:在2013和2014年哈尔滨、克山3个环境下定位了控制大豆全生育期的14个QTL,分别定位在大豆20个连锁群中的A2、B2、C1、C2、D1b、E、F、G、K、L、N共11个连锁群上,遗传率为1.34%~9.19%。可延长全生育期的优异等位基因型有BARCSOYSSR_08_0966(Q3Q3)、Sat_177(Q2Q2)、Sct_186(Q3Q3)、Satt307(Q3Q3)、Satt557(Q1Q1)、Satt577(Q2Q2)、Sat_351(Q1Q1)、Satt268(Q1Q1)、Sct_199(Q1Q1)、Satt273(Q3Q3)、Satt229(Q1Q1)、Satt664(Q1Q1)、Satt125(Q4Q4),能够缩短全生育期的优异等位基因型有BARCSOYSSR_08_0966(Q2Q2)、Sat_177(Q1Q1)、Sct_186(Q1Q1)、Satt307(Q2Q2)、Satt557(Q2Q2)、Satt577(Q4Q4)、Sat_351(Q2Q2)、Satt268(Q3Q3)、Sct_199(Q3Q3)、Satt273(Q2Q2)、Satt229(Q3Q3)、Satt664(Q3Q3)、Satt125(Q3Q3)。Satt307、Satt199和Satt125这3个QTL在2个环境重复检测到,其中Satt199在两个环境中的遗传率和等位基因的遗传效应差异较小,受环境条件的影响较小,可用于分子设计育种。
Four-way recombinant inbred lines containing 160 lines were used as experimental materials to identify individual genotypes by using 188 SSR markers. Single-marker analysis was used to analyze the phenotypic data of the whole growth period Population QTLs were analyzed during the whole growth period. The results showed that in the three environments of Harbin and Keshan in 2013 and 2014, 14 QTLs controlling the whole growth period of soybean were located and were located in A2, B2, C1, C2, D1b, E, F, G, K, L, N, a total of 11 linkage groups, the heritability of 1.34% ~ 9.19%. Excellent alleles alleles of BARCSOYSSR_08_0966 (Q3Q3), Sat_177 (Q2Q2), Sct_186 (Q3Q3), Satt307 (Q3Q3), Satt557 (Q1Q1), Satt577 (Q2Q2), Sat_351 (Q1Q1) and Satt268 (Q1Q1), Satt273 (Q3Q3), Satt229 (Q1Q1), Satt664 (Q1Q1), and Satt125 (Q4Q4) in the whole growth period were significantly decreased (Q1Q1), Satt 307 (Q2Q2), Satt 557 (Q2Q2), Satt577 (Q4Q4), Sat_351 (Q2Q2), Satt 268 (Q3Q3), Sct_199 (Q3Q3), Satt 273 (Q2Q2), Satt229 (Q3Q3), Satt664 (Q3Q3) (Q3Q3). The three QTLs Satt307, Satt199 and Satt125 were repeatedly detected in two environments. Among them Satt199 had less genetic heredity and allelic genetic effects in two environments, less affected by environmental conditions, and could be used in molecular design Breeding.