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抑癌基因p53具有广谱抑癌作用，该基因失活是细胞发生癌变的重要因素。p53基因失活的机制是多种的，其中突变是其失活的主要原因之一〔1〕。第7外显子是p53基因突变的热点区之一，在黄曲霉毒素高污染区的肝癌存在该外显子第249位密码子特异突变〔2〕。本实验应用PCR－SSCP同位
The tumor suppressor gene p53 has a broad-spectrum anti-cancer effect. Inactivation of this gene is an important factor in canceration of the cell. The mechanism of p53 gene inactivation is many, and mutations are one of the main reasons for its inactivation [1]. Exon 7 is one of the hotspots of the p53 gene mutation. There is a 249th codon-specific mutation in this exon in the high-contamination area of aflatoxin [2]. This experiment uses PCR-SSCP homology