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植物中存在许多多效性基因,它们在调控植物的营养生长与生殖发育过程中起着关键性作用。文章在籼稻育种材料中发现了一个植株显著矮化且花器官明显变异的突变体ddf1(dwarf and deformed flower 1)。遗传分析表明,该突变体由单基因隐性突变所致,这说明该基因是一个同时控制营养生长和生殖发育的多效性基因,暂命名为DDF1。为了定位该基因,将ddf1杂合体与热带粳稻品种DZ60杂交,建立了F2定位群体,利用水稻RM系列微卫星标记,通过混合分离分析(BSA)和小群体连锁分析,将DDF1初步定位在水稻第6号染色体RM588和RM587标记之间,与两标记的遗传距离分别为3.8 cM和2.4 cM。进一步利用已经公布的水稻基因组序列,在初步定位的区间内开发新的SSR标记,将DDF1定位在165 kb的区间内。该结果为克隆DDF1奠定了基础。
There are many pleiotropic genes in plants, which play key roles in the regulation of plant vegetative and reproductive development. In the present study, a mutant dwarf and deformed flower 1 with significantly dwarfed plants and obvious flower organ was found in indica rice. Genetic analysis showed that the mutant was caused by a recessive single gene mutation, indicating that the gene is a pleiotropic gene that controls both vegetative and reproductive development and is tentatively named DDF1. In order to locate the gene, the F2 population was crossed with the hybrid of the ddf1 hybrid and the tropical japonica rice variety DZ60. Based on the RM series of microsatellite markers in rice, by means of mixed segregation analysis (BSA) and small group linkage analysis, DDF1 was initially mapped in rice The genetic distance between markers RM588 and RM587 on chromosome 6 and the two markers were 3.8 cM and 2.4 cM, respectively. Further exploiting the published rice genome sequence, a new SSR marker was developed within the initial mapping interval, and DDF1 was located within a 165 kb interval. This result laid the foundation for the cloning of DDF1.