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本研究用ABC免疫组织化学法(免疫组化法)研究了9例慢性活动性肝炎(CAH)患者肝组织内肿瘤坏死因子—α(TNF—α)含量;同时测定血清及外周血单个核细胞(PBMC)的TNF—α含量及肝组织学活动指数(HAI)分数。结果显示TNF—α单抗主要染色浸入肝组织内的单个核细胞(mononuclear cells,MNCs);部分肝细胞也染色阳性。肝内TNF—α阳性细胞MNCs数量与肝组织HAI分数和PBMC的TNF—α水平呈正相关。提示肝组织内的TNF—α主要由MNCs产生;肝脏内TNF—α水平可以反映肝损害的严重程度。肝组织内异常升高的TNF—α水平可导致免疫病理反应,介调肝细胞损害。
In this study, ABC immunohistochemistry (immunohistochemical method) to study the nine patients with chronic active hepatitis (CAH) in patients with liver tumor necrosis factor-α (TNF-α) content; simultaneous determination of serum and peripheral blood mononuclear cells (PBMC) TNF-α content and liver histology activity index (HAI) score. The results showed that the main staining of TNF-α mAb was immersed in mononuclear cells (MNCs) in liver tissue; some of the hepatocytes were also stained positive. The number of intrahepatic TNF-α-positive MNCs was positively correlated with the level of HAI in liver tissue and TNF-α in PBMC. It is suggested that TNF-α in liver tissue is mainly produced by MNCs. The level of TNF-α in liver can reflect the severity of liver damage. Abnormally elevated levels of TNF-α in liver tissue can lead to immunopathological responses mediated by hepatocellular damage.